VIASURE E. coli Typing Real Time PCR Detection Kit is designed for the specific identification and differentiation of Enterohemorrhagic Escherichia coli (EHEC), Shiga toxin-producing Escherichia coli (STEC), Enteropathogenic Escherichia coli (EPEC), Enterotoxigenic Escherichia coli (ETEC) and Enteroinvasive Escherichia coli (EIEC)/Shigella in human stool samples from patients with signs and symptoms of gastrointestinal infection.
This test is intended to be used as an aid in the diagnosis of EHEC, STEC, EPEC, ETEC and EIEC/Shigella in combination with clinical and epidemiological risk factors.
DNA is extracted from stool specimens, multiplied using Real Time amplification and detected using specific primers and a fluorescent reporter dye probe for EHEC, STEC, EPEC, ETEC and EIEC/Shigella.
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